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在线活细胞浓度在昆虫杆状病毒表达系统sf9中的应用
  • 发布日期:2014-09-19      浏览次数:4436
    • Animal cells Sf9动物细胞sf9

      活细胞浓度分析仪  订购:    索取详细资料:

      Sf9 Insect cell growth and infection process by a baculovirus (BEVS)
      sf9昆虫细胞的生长和感染的病毒进程(BEVS
      The FOGALE BIOMASS SYSTEM signal provides    FOGALE BIOMASS 活细胞浓度分析仪提供实时数据信号
      direct real time informations on: 直接的实时信息

      >Cell growth monitoring: 细胞生长监测:
      The permittivity signal is correlated to viable cell count during the growth phase.

      在细胞的生长阶段电容信号与活细胞数有正相关性。
      >Time of infection: 感染时刻
      Cells are infected while they are in logarithm growth phase, the time of infection that is of great importance for productivity is accuretly determined.

      细胞受到感染的同时处于对数生长期,确定准确的感染时间对于生产来说至关重要!
      >Success of baculovirus infection: 杆状病毒感染的成功
      The plateau region in the permittivity profile (at 12 to 20h p.i.) has been related as an indicator of infection synchronicity (Zeiser et al, 2000)
      在介电容值的波峰区(在1220hπ)与细胞感染的同步(Zeiser et al, 2000
      >Harvest time: 产出
      It is of crucial importance for the yield and quality of the recombined protein.

      这对重组蛋白的产量和质量来说至关重要。
      The cell lysis monitoring helps in determining the optimal harvest time during the release phase.

      细胞凋亡裂解的实时监测有助于释放阶段确定*采收期

      Permittivity, biovolume in a batch culture of insect cell infected by a baculovirus.

      电容,生物量在单次感染病毒的昆虫细胞

      Permittivity, viable cell count (trypan blue exclusion method), cell diameter (CASY counter)

      电容,活细胞数(台盼蓝不相容试验),细胞直径(CASY计数器)